Evolution of the Immune Landscape in the Progression of Gynecological Cancers
The prevalent gynecologic cancers, endometrial and ovarian, are the focus of our study aimed to unveil immune dynamics in their development, diagnosis, and progression.
Employing tissue-engineered cell-based in vitro models that mimic many aspects of the complex endometrial and ovarian tumor microenvironment, we systematically investigate the immune response in correlation with the tumor stage. This analysis involves dissecting phenotypic, effector and transcriptional changes through combination of flow cytometry and RNA/TCR-seq technics, complemented by advanced biostatistician tools, encompassing cutting-edge algorithms and statistical models.
Our approach seeks to advance diagnostic precision and facilities the development of personalized immunotherapies for gynecological malignances.
TEAM
Joanna Mikulak PhD - Senior staff scientist
Valentina Cazzetta - Post-doctoral fellow
Paolo Marzano - PhD student, bioinformatician
Annalisa Imperiali - PhD student, bioinformatician
Anna Carletti – Lab manager
collaborations
Gynecology Unit
IRCCS Humanitas Research Hospital, Milan, Italy
Unit of Gynecological Oncology Research
IEO, Milan, Italy
Cancer Pharmacology Lab
IRCCS Humanitas Research Hospital, Milan, Italy
Pathology Unit
IRCCS Humanitas Research Hospital, Rozzano, Milan
Dipartimento di Medicina Sperimentale
Università degli Studi di Genova, Genova, Italy
related publications
Bernson E, Huhn O, Karlsson V, Hawkes D, Lycke M, Cazzetta V, Mikulak J, Hall J, Piskorz AM, Portuesi R, Vitobello D, Fiamengo B, Siesto G, Horowitz A, Ghadially H, Mavilio D, Brenton JD, Sundfeldt K, Colucci F.
Cancers (Basel). 2023 Jun 27;15(13):3362. doi: 10.3390/cancers15133362.NKG2A Immune Checkpoint in Vδ2 T Cells: Emerging Application in Cancer Immunotherapy
Cazzetta V, Depierreux D, Colucci F, Mikulak J, Mavilio D.
Cancers (Basel). 2023 Feb 16;15(4):1264. doi: 10.3390/cancers15041264.Di Vito C, Mikulak J, Zaghi E, Pesce S, Marcenaro E, Mavilio D.
Semin Immunol. 2019 Feb;41:101272. doi: 10.1016/j.smim.2019.03.004.Brummelman J, Mazza EMC, Alvisi G, Colombo FS, Grilli A, Mikulak J, Mavilio D, Alloisio M, Ferrari F, Lopci E, Novellis P, Veronesi G, Lugli E.
J Exp Med. 2018 Oct 1;215(10):2520-2535. doi: 10.1084/jem.20180684. Epub 2018 Aug 28. PMID: 30154266; PMCID: PMC6170179.